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dc.contributor.authorTunca, Berrin
dc.contributor.authorTezcan, Gülçin
dc.contributor.authorÇeçener, Gülşah
dc.contributor.authorEgeli, Ünal
dc.contributor.authorAk, Seçil
dc.contributor.authorMalyer, Hulusi
dc.contributor.authorTümen, Gülendam
dc.contributor.authorBilir, Ayhan
dc.date.accessioned2019-08-02T13:43:45Z
dc.date.available2019-08-02T13:43:45Z
dc.date.issued2012en_US
dc.identifier.urihttps://doi.org/10.1007/s00432-012-1261-8
dc.identifier.urihttps://hdl.handle.net/20.500.12462/5769
dc.descriptionTümen, Gülendam (Balikesir Author)en_US
dc.description.abstractGlioblastoma multiforme (GBM) is the most common and the most lethal form of primary malignant tumors in the central nervous system. There is an increasing need for the development of more efficient therapeutic approaches for the treatment of these patients. One of the most attractive cancer therapy methods to date is the induction of tumor cell death by certain phytochemicals. Interestingly, bioactive compounds have been shown to alter micro RNA (miRNA) expression involved in several biological processes at the posttranscriptional level. The present study aimed to evaluate whether Olea europaea leaf extract (OLE) has an anticancer effect and modulates miRNA expression in GBM. Firstly, the anti-proliferative activity of OLE and the nature of the interaction with temozolomide (TMZ) of OLE were tested in human glioblastoma cell line T98G cells by trypan blue and WST-1 assays and than realized miRNA PCR array analysis. Potential mRNA targets were analyzed bioinformatically. OLE exhibited anti-proliferative effects on T98G cell lines. Cells were treated with temozolomide (TMZ) in the presence OLE, and changes to miRNA expression levels were identified by PCR array analysis. miRNA target genes are involved in cell cycle and apoptotic pathways. Specifically, miR-181b, miR-153, miR-145, miR-137, and let-7d were significantly upregulated after treatment with both TMZ and OLE. Our results suggest that OLE modulates the expression of some miRNAs related to anticancer activity in GBM and the response to TMZ. Further studies and validations are needed, but we suggest that OLE might be used for in vivo studies and future medical drug studies.en_US
dc.description.sponsorshipUludag University - UAP (T)-2010/7en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s00432-012-1261-8en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectOlea Europaea Leaf Extracten_US
dc.subjectTemozolomideen_US
dc.subjectGlioblastoma Multiformeen_US
dc.subjectMicrornaen_US
dc.titleOlea europaea leaf extract alters microRNA expression in human glioblastoma cellsen_US
dc.typearticleen_US
dc.relation.journalJournal of Cancer Research and Clinical Oncologyen_US
dc.contributor.departmentFen-Edebiyat Fakültesien_US
dc.contributor.authorID0000-0001-7904-883Xen_US
dc.contributor.authorID0000-0002-5956-8755en_US
dc.identifier.volume138en_US
dc.identifier.issue11en_US
dc.identifier.startpage1831en_US
dc.identifier.endpage1844en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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