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dc.contributor.authorGençer, Nahit
dc.contributor.authorYavuz, Emre
dc.date.accessioned2019-10-07T06:19:52Z
dc.date.available2019-10-07T06:19:52Z
dc.date.issued2017en_US
dc.identifier.issn1381-3455
dc.identifier.issn1744-4160
dc.identifier.urihttps://doi.org/ 10.1080/13813455.2017.1279632
dc.identifier.urihttps://hdl.handle.net/20.500.12462/6704
dc.description.abstractIn this study, an alternative purification method for human Paraoxonase 1 (hPON1) enzyme was developed using two-step procedures, namely ammonium sulphate precipitation and Sepharose-4B-L-tyrosine-1-aminoanthracene hydrophobic interaction chromatography. SDS-polyacrylamide gel electrophoresis of the enzyme indicates a single band with an apparent MW of 43 kDa. The enzyme was purified 674-fold with a yield of 16%. Furthermore, we examined the in vitro effect of methidathion on the enzyme activity to understand the better inhibitory properties of the compound. Methidathion is a highly toxic insecticide used to control a broad spectrum of agricultural insect and mite pests. IC50 value was found to be 0.130mM for the pesticide. Methidathion showed a competitive inhibition with Ki of 0.119mM for paraoxon.en_US
dc.description.sponsorshipBalikesir University Research Project - 2013/30en_US
dc.language.isoengen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.isversionof10.1080/13813455.2017.1279632en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectPON1en_US
dc.subjectHydrophobic Interaction Chromatographyen_US
dc.subjectPurificationen_US
dc.subjectInhibitionen_US
dc.subjectMethidathionen_US
dc.titleAn alternative purification method for human serum paraoxonase 1 and its interaction with methidathionen_US
dc.typearticleen_US
dc.relation.journalArchives of Physiology and Biochemistryen_US
dc.contributor.departmentFen Edebiyat Fakültesien_US
dc.contributor.authorID0000-0001-7092-8857en_US
dc.identifier.volume123en_US
dc.identifier.issue3en_US
dc.identifier.startpage159en_US
dc.identifier.endpage164en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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