Investigation of antibacterial effects of Zizyphus jujuba
AuthorÖzkan, Halil İbrahim
Yavuz, Mehmet Tevfik
Hişmioğulları, Adnan Adil
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Plants are most important parts of natural resources that alterna-tively referred to synthetic drugs for reasons such as being lessside effects and lower costs.Ziziphus jujuba Miller(Z. jujuba), aplant used in traditional medicine, is one of the most importantZiziphus species belonging to Rhamnacea family. The fruit andseeds of this plant are used different purposes such as anti-inflammatory, antioxidant, immune-stimulant and wound healer.In this study, we investigated the antibacterial effects ofZ.jujuba.The aims of this study were to screen the antibacterial activityofZ. jujuba. The extract was obtained fromZ. jujubafruits pul-verized with the aid of ball mill using 50% aqueous-ethanol solu-tion. Extracts were screened for antimicrobial activity against sixdifferent standard strains of bacteria by determining minimuminhibitory concentration (MIC) according to CLSI criteria. Serialdilutions are made between 64 mg/ml and 0.031 mg/ml concen-tration range. The lowest concentration of wells that no visiblegrowth has been accepted as MIC value. Materials in the MICand lower concentrated wells were transferred to 5% SheepBlood Agar petri dishes for calculation of minimal bactericidalconcentration (MBC). The lowest concentration that no colonyformation has been accepted as MBC value.jujubashowed the most potent effect on strain ofS. aureusATCC 29213 is Gram-positive cocci (MIC: 2 mg/ml). The MICvalues of other Gram-positive bacteriaS. aureusATCC 43300,E. faecalisATCC 51219,L. monocytogenesF 1483 andM. smeg-matisCMM 2067 were detected as 8, 16, 8 and 8 mg/ml respec-tively. MIC values of Gram-negative bacilli were detected as>64 mg/ml.Consequently,Z. jujubawas found to be effective on Gram-positive cocci bacteria (S. aureusATCC 29213,S. aureusATCC 43300 andE. faecalisATCC 51219). The strongest effect wasobserved onS. aureusATCC 29213 strain. In contrast, extractshowed less effect on Gram-negative bacilli.