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dc.contributor.authorDedeoğlu, Nurcan
dc.contributor.authorDe Luca, Viviana
dc.contributor.authorIşık, Semra
dc.contributor.authorYıldırım, Hatice
dc.contributor.authorKöçkar, Feray
dc.contributor.authorCapasso, Clemente
dc.contributor.authorSupuran, Claudiu T.
dc.date.accessioned2019-10-17T07:56:11Z
dc.date.available2019-10-17T07:56:11Z
dc.date.issued2015en_US
dc.identifier.issn0968-0896
dc.identifier.issn1464-3391
dc.identifier.urihttps://doi.org/10.1016/j.bmc.2015.05.007
dc.identifier.urihttps://hdl.handle.net/20.500.12462/7784
dc.descriptionDedeoğlu, Nurcan (Balikesir Author)en_US
dc.description.abstractThe oral pathogenic bacterium involved in human dental caries formation Streptococcus mutans, encodes for two carbonic anhydrase (CA, EC 4.2.1.1) one belonging to the alpha- and the other one to the beta-class. This last enzyme (SmuCA) has been cloned, characterized and investigated for its inhibition profile with a major class of CA inhibitors, the inorganic anions. Here we show that SmuCA has a good catalytic activity for the CO2 hydration reaction, with k(cat) 4.2 x 10(5) s (1) and k(cat)/K-m of 5.8 x 10(7) M (1) x s (1), being inhibited by cyanate, carbonate, stannate, divannadate and diethyldithiocarbamate in the submillimolar range (K(I)s of 0.30-0.64 mM) and more efficiently by sulfamide, sulfamate, phenylboronic acid and phenylarsonic acid (K(I)s of 15-46 mu M). The anion inhibition profile of the S. mutans enzyme is very different from other alpha- and beta-CAs investigated earlier. Identification of effective inhibitors of this new enzyme may lead to pharmacological tools useful for understanding the role of S. mutans CAs in dental caries formation, and eventually the development of pharmacological agents with a new mechanism of antibacterial action.en_US
dc.language.isoengen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.isversionof10.1016/j.bmc.2015.05.007en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectCarbonic Anhydraseen_US
dc.subjectBeta-Class Enzymeen_US
dc.subjectAnion Inhibitoren_US
dc.subjectStreptococcus Mutansen_US
dc.titleCloning, characterization and anion inhibition study of a beta-class carbonic anhydrase from the caries producing pathogen streptococcus mutansen_US
dc.typearticleen_US
dc.relation.journalBioorganic & Medicinal Chemistryen_US
dc.contributor.departmentFen Edebiyat Fakültesien_US
dc.contributor.authorID0000-0003-3314-2411en_US
dc.contributor.authorID0000-0003-2572-8391en_US
dc.identifier.volume23en_US
dc.identifier.issue13en_US
dc.identifier.startpage2995en_US
dc.identifier.endpage3001en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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