Browsing by Author "Güler, Özen Özensoy"
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Bazı karbonik anhidraz izoenzimlerinin saflaştırılması için yeni bir afinite jelinin sentezi ve uygulanması
Şahin, Ayşegül (Balıkesir Üniversitesi Fen Bilimleri Enstitüsü, 2008)Afinite kromatografisi, proteinlerin saflaştırılmasında, kullanışlı ve uygun bir tekniktir. Bu teknik ile ligand olarak daha önceden orijinal olarak sentezlenmiş bir CA inhibitörü 4-izosiyonatbenzensülfonamit, çalışmada ... -
Carbonic anhydrase activators: Activation of the β-carbonic anhydrase Nce103 from the yeast Saccharomyces cerevisiae with amines and amino acids
Işık, Semra; Köçkar, Feray; Aydın, Meltem; Arslan, Oktay; Güler, Özen Özensoy; Innocenti, Alessio; Scozzafava, Andrea; Supuran, Claudiu T. (Pergamon-Elsevier Science Ltd, 2009)The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, was investigated for its activation with amines and amino acids. scCA was poorly activated ... -
Carbonic anhydrase inhibitors. Inhibition of the beta-class enzyme from the yeast Saccharomyces cerevisiae with anions
Işık, Semra; Köçkar, Feray; Arslan, Oktay; Güler, Özen Özensoy; Innocenti, Alessio; Supuran, Claudiu T. (Pergamon-Elsevier Science Ltd, 2008)The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, has been cloned, purified, characterized kinetically, and investigated for its inhibition ... -
Carbonic anhydrase inhibitors: Inhibition of the β-class enzyme from the yeast Saccharomyces cerevisiae with sulfonamides and sulfamates
Işık, Semra; Köçkar, Feray; Aydın, Meltem; Arslan, Oktay; Güler, Özen Özensoy; Innocenti, Alessio; Scozzafava, Andrea; Supuran, Claudiu T. (Pergamon-Elsevier Science Ltd, 2009)The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase ( CA, EC 4.2.1.1) designated as scCA, has been cloned, purified, characterized kinetically and investigated for its inhibition ... -
Catalase, carbonic anhydrase and xanthine oxidase activities in patients with mycosis fungoides
Cengiz, Fatma Pelin; Beyaztaş, Serap; Gökçe, Başak; Arslan, Oktay; Güler, Özen Özensoy (Taylor & Francis Ltd, 2014)Mycosis fungoides (MF) is the most common form of cutaneous T-cell lymphoma. In several studies the relationship between catalase (CAT), human cytosolic carbonic anhydrases (CA; hCA-I and hCA-II) and xanthine oxidase (XO) ... -
Characterization of polyphenoloxidase from wild pear (Pyrus elaegrifolia)
Yerlitürk, Fatma Ülker; Arslan, Oktay; Sinan, Selma; Gençer, Nahit; Güler, Özen Özensoy (Wiley, 2008)Wild pear polyphenoloxidase (PePPO) was extracted and purified using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column. Optimum conditions for pH, temperature and heat inactivation were determined. At the ... -
Differential in vitro inhibition of polyphenoloxidase from a wild edible mushroom Lactarius salmonicolor
Dedeoğlu, Nurcan; Güler, Özen Özensoy (Taylor & Francis Ltd, 2009)The polyphenol oxidase (LsPPO) from a wild edible mushroom Lactarius salmonicolor was purified using a Sepharose 4B-L-tyrosine-p-amino benzoic acid affinity column. At the optimum pH and temperature, the KM and VMax values ... -
Differential in vitro inhibition studies of some cerium vanadate derivatives on xanthine oxidase
Kaya, Mustafa Oğuzhan; Kaya, Yeşim; Gül, Gülşah Çelik; Kurtuluş, Figen; Arslan, Oktay; Güler, Özen Özensoy (Informa Healthcare, 2015)In this preliminary study, a new series of some cerium vanadate derivatives have been investigated as new type of inhibitors of xanthine oxidase (XO; E.C 1.17.3.2). XO is a superoxide-producing enzyme found normally in ... -
Differential in vitro inhibitory effects of anticancer drugs on tumor-associated carbonic anhydrase isozymes CA IX and CA XII
Güler, Özen Özensoy; Arslan, Oktay; Köçkar, Feray (Prous Science, 2008)Carbonic anhydrase IX (CA IX) and, to a lesser extent. carbonic anhydrase XII (CA XII) are highly overexpressed in hypoxic tumors. In this study, the inhibitory effects of 11 different anticancer drugs including paclitaxel, ... -
Drug design studies of the novel antitumor targets carbonic anhydrase IX and XII
Güler, Özen Özensoy; De Simone, Giovanni; Supuran, Claudiu T. (Bentham Science Publ Ltd, 2010)The carbonic anhydrase (CA, EC 4.2.1.1) isozymes IX and XII are predominantly found in tumor cells and show a restricted expression in normal tissues. By efficiently hydrating carbon dioxide to protons and bicarbonate, ... -
Effects of some adsorbents on the pre-purification of taxol (anticancer drug) from hazelnut nutshells
Oğuzkan, Sibel Bayıl; Karadeniz, Şeref; Karagül, Bora; Uzun, Ayşe; Aksoy, Elif Sine; Güler, Özen Özensoy; Çakır, Ümit; Ugraş, Halil İbrahim (Asian Network Scientific Information-Ansinet, 2018)Background and Objective: The most important pharmaceutically active substance in plant-derived chemotherapy drugs is paclitaxel. Taxane compounds such as paclitaxel, cephalomannine, baccatin III and 10-Deacetylbaccatin ... -
Effects of some antibiotics on enzyme activities of carbonic anhydrase from erythrocytes in vivo and in vitro
In this study, firstly, the effects of eight antibiotics namely, chlarithromycin, chloramphenicol, rifamycin SV, clindamycin phosphate, ciprofloxacin, gentamycin sulfate, sodium ampicillin and cefazolin sodium, on human ... -
In vitro efficacy of some cattle drugs on bovine serum paraoxonase 1 (PON1) activity
Arslan, Mikail; Gencer, Nahit; Arslan, Oktay; Güler, Özen Özensoy (Informa Healthcare, 2012)Serum paraoxonase 1 (EC 3.1.8.1, PON1), a calcium-associated enzyme, has an ability to hydrolyze organophosphate compounds. Related to this property, PON1 has a critical role in antioxidant mechanisms. It is well-known ... -
Investigation of heavy metal effects on immobilized paraoxanase by glutaraldehyde
Bilen, Çiğdem; Beyaztaş, Serap; Arslan, Oktay; Güler, Özen Özensoy (Informa Healthcare, 2013)Serum paraoxonase 1 (PON1) was purified from bovine serum using hydrophobic interaction chromotography on Sepharose 4B-coupled L-tyrosine 1-naphthylamine gel, and monitored by sodium dodecyl sulfate polyacrylamide gel ... -
Is there a relation between genetic susceptibility with cancer? A study about paraoxanase (PON1) enzyme activity in breast cancer cases
Kaya, Mustafa Oğuzhan; Sinan, Selma; Güler, Özen Özensoy; Arslan, Oktay (Taylor & Francis Ltd., 2016)Human serum paraoxonase 1 (PON1, EC 3.1.1.2) is a high density lipoprotein (HDL)-associated antioxidant enzyme that not only decreases oxidative stress, but it is also implicated in development of many cancers. Genetic ... -
Mutation of phe91 to asn in human carbonic anhydrase ı unexpectedly enhanced both catalytic activity and affinity for sulfonamide inhibitors
Köçkar, Feray; Maresca, Alfonso; Işık, Semra; Türkoğlu, Sümeyye; Sinan, Selma; Arslan, Oktay; Güler, Özen Özensoy; Turan, Yusuf (Pergamon-Elsevier Science Ltd, 2010)Site-directed mutagenesis has been used to change one amino acid residue considered non essential (Phe91Asn) to catalysis in carbonic anhydrase (CA, EC 4.2.1.1) isozyme I (hCA I), but which is near the substrate binding ... -
A new affinity gel for the purification of alpha-carbonic anhdrases
Şahin, Ayşegül; Işık, Semra; Arslan, Oktay; Supuran, Claudiu T.; Güler, Özen Özensoy (Taylor & Francis Ltd, 2015)The new affinity gel reported in this study was prepared using EUPERGIT C250L as a chromatographic bed material, to which etylenediamine spacer arms were attached to prevent steric hindrance between the matrix and ligand, ... -
A new affinity method for purification of bovine testicular hyaluronidase enzyme and an investigation of the effects of some compounds on this enzyme
Terzi, Emine; Kaya, Mustafa Oğuzhan; Arslan, Oktay; Güler, Özen Özensoy (Wiley-Blackwell, 2015)In this study, a new affinity gel for the purification of bovine tes-ticular hyaluronidase (BTH) was synthesized. L-Tyrosine wasadded as the extension arm to the Sepharose-4B activated withcyanogen bromide. m-Anisidine is ... -
A new affinity method for purification of bovine testicular hyaluronidase enzyme and an investigation of the effects of some compounds on this enzyme
Kaya, Mustafa Oğuzhan; Arslan, Oktay; Güler, Özen Özensoy (Taylor & Francis Ltd, 2015)In this study, a new affinity gel for the purification of bovine testicular hyaluronidase (BTH) was synthesized. L-Tyrosine was added as the extension arm to the Sepharose-4B activated with cyanogen bromide. m-Anisidine ... -
Nükleik asit tayinine yönelik elektrokimyasal genosensör dizaynı
Bozdağ, Murat (Balıkesir Üniversitesi Fen Bilimleri Enstitüsü, 2010)Günümüzde, LNA probların elektrokimyasal hibridizasyon analizi geliştirmesinde kullanımı giderek artmaktadır. RNA benzeri bir nükleik asit olan LNA, riboz şekerinin furanoz halkasının kimyasal olarak 2´oksijeni ile 4´ ...
