| dc.description.abstract | In this study, the phylogenetic relationship of the endemic Sideritis taxa, Sideritis brevidens P.H.Davis, Sideritis congesta P.H.Davis & Hub.-Mor., Sideritis erythrantha var. cedretorum P.H.Davis, Sideritis libanotica subsp. violascens (P.H.Davis) P.H.Davis, Sideritis rubriflora Hub.-Mor, and Sideritis vuralii H.Duman & Ba & scedil;er that are distributed in Mersin province in T & uuml;rkiye, determined using chloroplast DNA (cpDNA) trnL-F, rbcL, and matK markers. After plant sampling, genomic DNA was isolated and PCR amplification was performed using the trnL-F, rbcL, and matK primers. The sequences were edited using Sequencher 5.4.6. Using MEGA 6.0 software, nucleotide ratios, genetic distance matrix, and pi = nucleotide diversity were obtained. Maximum likelihood (ML) phylogenetic trees were generated using some Sideritis and Stachys L. species from National Center for Biotechnology Information (NCBI). In the past, several investigations have been carried out on the phylogenetic position of Sideritis and Stachys species. We have determined the phylogenetic position of the endemic Sideritis species using the sequences of some other Sideritis and Stachys taxa. As a result, the highest nucleotide diversity was detected in matK analysis (0.006698) and the lowest was detected in trnL-F analysis (0.001305). While our species were found in the same group with Sideritis and Stachys species in the rbcL analysis, in trnL-F analyses, our endemic Sideritis species Sideritis syriaca and Sideritis scardica appeared in a group. In our matK analyses, only our endemic Sideritis species appeared together and were supported by a 98% bootstrap value. In this study, the trnL-F, rbcL and matK sequences did not generally provide a high degree of resolution in the species and subspecies categories. While the trnL-F and matK results showed that our Sideritis species were monophyletic, our rbcL results showed that they were not. The results of this study will provide references for future phylogenetic analyses of Sideritis species and related taxa. | en_US |
