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dc.contributor.authorSağkan, Rahşan İlikçi
dc.contributor.authorKöçkar, Feray
dc.contributor.authorŞengül, A. Li
dc.contributor.authorYılmaz, Sibel
dc.contributor.authorMüsabak, Uğur Hacı
dc.date.accessioned2019-10-16T11:39:48Z
dc.date.available2019-10-16T11:39:48Z
dc.date.issued2011en_US
dc.identifier.issn1742-464X
dc.identifier.urihttps://hdl.handle.net/20.500.12462/7161
dc.descriptionKöçkar, Feray (Balikesir Author)en_US
dc.description.abstractCarbonic anhydrase IX (CAIX) is a transmembraneenzyme associated with tumor metabolism. Several studies impli-cated that CAIX could be a prognostic marker in several type oftumor. Therefore, the detection of protein is valuable for clinicalstudies. In this study, the protein expression of Carbonic anhydr-ase IX was analysed by flow cytometry in adherent colon carci-noma cells, namely HT-29. In order to optimise the detection ofHT-29 cells, several parametres have been assessed. Sample prep-aration that means produce a suspension of single cells is a veryimportant experimental stage in flow cytometry. Two differentmethods was used for the preparation of cells to flow cytometry,namely chemical methods Tripsin-EDTA and physical method,cell scrabers tecniques. PE staining Human CAIX monoclonalantibody was used for the detection. . According to the resultsobtained from this optimization study, cell suspension prepara-tion by cell scraper resulted in the more significantly CAIX pro-tein expression in HT-29 cells than chemical Tripsin-EDTAmethod. This could be that CAIX that is a membrane protein islikely to being damaged by Tripsin-EDTA method during thetreatment.en_US
dc.language.isoengen_US
dc.publisherWiley-Blackwellen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCarbonic Anhydrase IXen_US
dc.subjectFlow Cytometryen_US
dc.subjectHT-29en_US
dc.subjectCell Scraperen_US
dc.subjectTripsin-EDTAen_US
dc.titleAnalysis of tumor associated transmembrane enzyme CAIX in HT-29 cells by flow cytometryen_US
dc.typeotheren_US
dc.relation.journalFebs Journalen_US
dc.contributor.departmentFen Edebiyat Fakültesien_US
dc.identifier.volume278en_US
dc.identifier.startpage211en_US
dc.identifier.endpage211en_US
dc.relation.publicationcategoryDiğeren_US


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