dc.contributor.author | Sağkan, Rahşan İlikçi | |
dc.contributor.author | Köçkar, Feray | |
dc.contributor.author | Şengül, A. Li | |
dc.contributor.author | Yılmaz, Sibel | |
dc.contributor.author | Müsabak, Uğur Hacı | |
dc.date.accessioned | 2019-10-16T11:39:48Z | |
dc.date.available | 2019-10-16T11:39:48Z | |
dc.date.issued | 2011 | en_US |
dc.identifier.issn | 1742-464X | |
dc.identifier.uri | https://hdl.handle.net/20.500.12462/7161 | |
dc.description | Köçkar, Feray (Balikesir Author) | en_US |
dc.description.abstract | Carbonic anhydrase IX (CAIX) is a transmembraneenzyme associated with tumor metabolism. Several studies impli-cated that CAIX could be a prognostic marker in several type oftumor. Therefore, the detection of protein is valuable for clinicalstudies. In this study, the protein expression of Carbonic anhydr-ase IX was analysed by flow cytometry in adherent colon carci-noma cells, namely HT-29. In order to optimise the detection ofHT-29 cells, several parametres have been assessed. Sample prep-aration that means produce a suspension of single cells is a veryimportant experimental stage in flow cytometry. Two differentmethods was used for the preparation of cells to flow cytometry,namely chemical methods Tripsin-EDTA and physical method,cell scrabers tecniques. PE staining Human CAIX monoclonalantibody was used for the detection. . According to the resultsobtained from this optimization study, cell suspension prepara-tion by cell scraper resulted in the more significantly CAIX pro-tein expression in HT-29 cells than chemical Tripsin-EDTAmethod. This could be that CAIX that is a membrane protein islikely to being damaged by Tripsin-EDTA method during thetreatment. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Wiley-Blackwell | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Carbonic Anhydrase IX | en_US |
dc.subject | Flow Cytometry | en_US |
dc.subject | HT-29 | en_US |
dc.subject | Cell Scraper | en_US |
dc.subject | Tripsin-EDTA | en_US |
dc.title | Analysis of tumor associated transmembrane enzyme CAIX in HT-29 cells by flow cytometry | en_US |
dc.type | other | en_US |
dc.relation.journal | Febs Journal | en_US |
dc.contributor.department | Fen Edebiyat Fakültesi | en_US |
dc.identifier.volume | 278 | en_US |
dc.identifier.startpage | 211 | en_US |
dc.identifier.endpage | 211 | en_US |
dc.relation.publicationcategory | Diğer | en_US |