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dc.contributor.authorŞahin, Ayşegül
dc.contributor.authorIşık, Semra
dc.contributor.authorArslan, Oktay
dc.contributor.authorSupuran, Claudiu T.
dc.contributor.authorGüler, Özen Özensoy
dc.date.accessioned2019-10-17T10:37:18Z
dc.date.available2019-10-17T10:37:18Z
dc.date.issued2015en_US
dc.identifier.issn1475-6366
dc.identifier.issn1475-6374
dc.identifier.urihttps://doi.org/10.3109/14756366.2014.912215
dc.identifier.urihttps://hdl.handle.net/20.500.12462/8187
dc.description.abstractThe new affinity gel reported in this study was prepared using EUPERGIT C250L as a chromatographic bed material, to which etylenediamine spacer arms were attached to prevent steric hindrance between the matrix and ligand, and to facilitate effective binding of the CA-specific ligand, of the aromatic sulfonamide type for the purification of alpha-carbonic anhydrases (Cas; EC 4.2.1.1). Indeed, the aminoethyl moieties of the affinity gel were derivatized by reaction with 4-isothiocyanatobenzenesulfonamide, with the formation of a thiourea-based gel, having inhibitory effects against CAs. Both bovine erythrocyte carbonic anhydrase BCA and human (h) erythrocyte CA isoforms I, II (hCA I and II) have been purified from hemolysates, by using this affinity gel. The greatest purification fold and column yields for BCA and for cytosolic (hCA I + II) enzymes were of 181-fold (21.07%) and 184-fold (9.49%), respectively. Maximum binding was achieved at 15 degrees C and I = 0.3 ionic strength for alpha-carbonic anhydrases.en_US
dc.description.sponsorshipŞahin, Ayşegül (Balikesir Author)en_US
dc.language.isoengen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.isversionof10.3109/14756366.2014.912215en_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectAffinity Chromatographyen_US
dc.subjectCarbonic Anhydraseen_US
dc.subjectPurificationen_US
dc.titleA new affinity gel for the purification of alpha-carbonic anhdrasesen_US
dc.typearticleen_US
dc.relation.journalJournal of Enzyme Inhibition and Medicinal Chemistryen_US
dc.contributor.departmentFen Edebiyat Fakültesien_US
dc.identifier.volume30en_US
dc.identifier.issue2en_US
dc.identifier.startpage224en_US
dc.identifier.endpage228en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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