Effect of etanercept, a tnf-alpha inhibitor, on depressive-like behavior in cafeteria diet-fed rats
Özet
Background: Obesity has been shown to be associated with depression. Recent studies have reported that obesity is not only as
a metabolic disorder but also as an inflammatory disease. In this
study we explored the effect of etanercept, a TNF-α inhibitor, on
depressive-like behavior in cafeteria diet (CD)-fed rats.
Methods: Six-week-old male Wistar Albino rats (300-350 g) were
divided into three groups (n=10): Control (not exposed to CD),
CD-fed (exposed to CD during 5 weeks) and CD-fed+etanercept
(exposed to CD and treated with etanercept (0.8 mg/kg/weekly/
subcutaneously during 5 weeks). CD was used to generate diet-induced obesity. The body weights of animals were measured
weekly. Forced swimming test (FST), the sucrose consumption and
preference test were used to investigate antidepressant effect of
etanercept. One-way ANOVA and Tukey’s post hoc test used for
statistical analysis.
Results: After 5 weeks, the body weight of CD-fed group was
higher than control group (p<0.001) and CD-fed+etanercept
group was lower than CD-fed group (p<0.05). In FST, there were
differences between three groups in terms of immobility time
during second day of testing (F(2,27)=8.174, p=0.017). CD-fed
group exhibited more immobility than control group (p<0.01), while
there was no difference between control and CD-fed+etanercept
group (p>0.05). Sucrose consumption and sucrose preference in
CD-fed group was lower than control group (p<0.05). However, the
behavior of rats in CD-fed+etanercept group did not differ from the
control group (p>0.05).
Conclusions: The results indicate the antidepressant effect of
etanercept on diet-induced obesity and TNF-α may play crucial
role on obesity-related mechanisms.
