Advanced Search

Show simple item record

dc.contributor.authorSarıoğlu, Nurhan
dc.contributor.authorSunay, Fatma Bahar
dc.contributor.authorYay, Arzu Hanım
dc.contributor.authorKorkut, Oğuzhan
dc.contributor.authorErel, Fuat
dc.contributor.authorHişmioğulları, Adnan Adil
dc.contributor.authorKöse, Mehmet
dc.contributor.authorYalçın, Betül
dc.date.accessioned2022-03-23T10:13:02Z
dc.date.available2022-03-23T10:13:02Z
dc.date.issued2021en_US
dc.identifier.issn1300-0144 - 1303-6165
dc.identifier.urihttps://doi.org/10.3906/sag-2010-303
dc.identifier.urihttps://hdl.handle.net/20.500.12462/12132
dc.descriptionSarıoğlu, Nurhan (Balikesir Author)en_US
dc.description.abstractBackground/aim: Acute lung injury (ALI) is a major cause of death in the intensive care unit. Lipopolysaccharide (LPS) induced lung injury is the most widely used experimental ALI model and provides opportunities for new targeting therapy. In this study, we investigated the effects of tocilizumab, adalimumab, and methylprednisolone in LPS-induced acute lung injury. Materials and methods: Lung injury was established by intratracheal instillation of LPS. The rats were randomly divided into six groups: LPS, control, and treatment groups (adalimumab, tocilizumab, methylprednisolone, adalimumab + tocilizumab). Bronchoalveolar lavage (BAL) and lung tissues were collected at 48 h and 96 h following LPS administration from each group. For histological analysis, hematoxylin–eosin (H&E) staining was performed. The sections were obtained for immunohistochemical analysis. IL-6 and TNF-alpha immunoreactivity were measured. Results: Intratracheal LPS application resulted in inflammatory cell infiltration of interstitial and alveolar spaces and thickening of the alveolar wall. All treatment groups showed significantly amelioration compared to LPS at 48 h. Interestingly, adalimumab and adalimumab + tocilizumab groups showed a significant amelioration of the lung histoarchitecture, compared to the prednisolone group at 96 h (p = 0.028, p = 0.025, respectively). Compared to the control group, LPS stimulation resulted in a significant increase in IL-6 and TNF-alpha immunoreactivity (p < 0.001). IL-6 and TNF-alpha expression were markedly reduced in all treatment groups at 48 h but the reduction was greater in the adalimumab and tocilizumab group than in the steroid. Administration with adalimumab and/or tocilizumab effectively decreased expression of TNF-alpha (p = 0.001) and IL-6 (p < 0.001) at 96 h, but prednisolone did not exert an effective decrease (p > 0.05). Conclusion: Adalimumab and/or tocilizumab significantly reduce the release of proinflammatory cytokines and improve the tissue inflammation in the experimental model of ALI. Our results suggest that adalimumab and/or tocilizumab have a more potent antiinflammatory effect on lung injury than the steroid.en_US
dc.description.sponsorshipBalikesir University Scientific Research Projects Coordination Unit 2014/16en_US
dc.language.isoengen_US
dc.publisherTubitak Scientific & Technical Research Council Turkeyen_US
dc.relation.isversionof10.3906/sag-2010-303en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subjectAdalimumaben_US
dc.subjectTocilizumaben_US
dc.subjectSteroiden_US
dc.subjectAcute Lung Injuryen_US
dc.titleAntiinflammatory effects of adalimumab, tocilizumab, and steroid on lipopolysaccharide-induced lung injuryen_US
dc.typearticleen_US
dc.relation.journalTurkish Journal of Medical Sciencesen_US
dc.contributor.departmentTıp Fakültesien_US
dc.contributor.authorID0000-0002-5180-9649en_US
dc.contributor.authorID0000-0002-2231-7979en_US
dc.contributor.authorID0000-0003-1176-8843en_US
dc.contributor.authorID0000-0002-3729-4108en_US
dc.contributor.authorID0000-0002-4788-8161en_US
dc.contributor.authorID0000-0001-9982-2714en_US
dc.contributor.authorID0000-0003-1176-8843en_US
dc.identifier.volume51en_US
dc.identifier.issue5en_US
dc.identifier.startpage2741en_US
dc.identifier.endpage2751en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/openAccess
Except where otherwise noted, this item's license is described as info:eu-repo/semantics/openAccess