The determination of effect of some antibiotics on paraoxonase 2 (PON2) enzyme activities in human macrophages cell

Abstract

The human paraoxonase (hPON) gene family includes threemembers: PON1, PON2, and PON3, located adjacent to eachother on the long arm of chromosome 7. The three hPONs shareapproximately 65% identity at the amino acid level and 70%identity at the nucleotide level. In humans, hPON1 and hPON3are primarily expressed in liver while expression of hPON3 is alsofound in kidney. In contrast, hPON2 is more widely expressedand is found in a variety of tissues including the heart, kidney,liver, lung, placenta, small intestine, spleen, stomach, and testis.The effect of PON2 enzyme that is strictly expressed in macro-phages. The aim of the study is to determine thein vitroeffect ofCefazolin sodium, Gentamycin sulphate, ampicillin sodium andChloramphenicol sodium succinate on PON2 enzyme in humanmacrophages cell, namely U937 cells. Two different substrateswere used for the determination enzyme activity of PON2, lactoseand phenylacetate Different concentrations of drug were used inorder to see any effect on PON2 enzyme. These antibiotics revealthe differential effects on PON2 enzyme in U937 macrophages atdifferent time points and concentrations of drugs.